The Sanyal lab studies mechanisms of protein synthesis in bacteria and the role of the ribosome in protein folding. We are particularly interested in understanding the role of GTP hydrolysis by translation factors, ribosome targeting antibiotics, ribosomal proteins, and various translation factors involved in termination and ribosome recycling. Our current focus is protein synthesis in mycobacteria, a pathogen causing tuberculosis. Using synthetic biology approach, we have developed a reconstituted transcription-translation-folding system (RTTF) that produces active proteins in the test tube. We have previously demonstrated protein folding activity of the ribosome (PFAR) and recently discovered a link between PFAR and prion diseases.
Read more in our popular science presentation or go directly to our ongoing research. Also read about Suparna Sanyal's research in the researcher profile at the webpage of Disciplinary domain of Science and Technology, Uppsala University.
JE 28 request
The purification of His-tagged ribosome and its subunits from E. coli JE28 using affinity chromatography can be easily scaled up for high-throughput ribosome purification without significant changes in the infrastructure. This method is quite cost effective both in terms of time and reagents, compared to other methods of ribosome purification and therefore can be employed for commercial purposes as well. Since the His-tagged ribosomes from JE28 are highly active in translation they will be useful in analytical functional assays as well as in cell-free protein synthesis systems.
Stop Codon Database
Here we provide a database on stop codon usage, covering more than 600 bacterial strains. The original publication contains more 4000 sequences, for convenience we list only a selection here. However, if you are interested in the complete database you can download it here. Feel free to use and modify our custom program, called STOP CODON COUNTER. If you have any questions regarding the database or the program feel free to contact us.
By using and or modifying the Stop Codon Counter you agree to the Creative Commons license.