Mechanisms and regulation of protein machines at the single-molecule and atomic level.
Molecular machines are proteins that convert chemical energy into conformational changes so as to carry out work in the cell. Our group studies how the structural architectures and dynamics of molecular machines together enable their function and regulation. One major focus are nucleic-acid interacting enzymes whose aberrant function or dysregulation is often associated with severe disease states such as cancer.
In order to understand the function and regulation of these enzymes at a molecular level, we develop and apply single-molecule fluorescence methodologies and combine them with a range of structural approaches, biochemistry and computer simulations.